2019-10-18The Maine Potato Disease Testing Laboratory (MPDTL) is an APHIS-accredited laboratory which began in 2004 as a partnership between the University of Maine and the Maine State Department of Agriculture In March of 2008the Maine Potato Board provided a new space for the MPDTL at 744 Main Street in Presque Isle On January 1 2010 under agreement Pathoscreen Pathoscreen kits for the diagnosis of plant pathogens are ELISA test kits that contain all the materials necessary to perform an ELISA protocol Most importantly our kits include precoated plates which save time and offer extraordinary stability Kits are available in

Plant Bacteria

2020-8-2Attention VALITEST TPS Participants: Our ToBRFV complete RNA PCR reaction kit will be validated during a Test Performance Study in the frame of the VALITEST project For ordering please follow the link below and please indicate VALITEST as order reference → 09175C/100 ToBRFV complete RNA PCR reaction kit

2018-7-31TaqMan RT-PCR Kit is designed for the detection of PSTVd specific RNA in a real-time PCR based on the use of TaqMan technology This kit is designed for research use only and not for use in diagnostic procedures Background Information The Potato Spindle Tuber Viroid (PSTVd) was the first viroid to be identified and the natural

Potato is widely grown in the EU as reported in the pest categorisation of non‐EU viruses and viroids of potato (EFSA PLH Panel 2020) 3 4 3 2 Climatic conditions affecting establishment Except for those conditions affecting survival of the host plants no eco‐climatic constrains exist for the PVM isolates categorised here

2020-7-31Description This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate Products using this method include but are not limited to P1709 P2088 P6140 P6782 P8125 P8170 P8250 P8375 P8415 and P8651 The continuous spectrophotometric rate determination (A 420 Light path = 1 cm) is based on the following reaction:

2019-8-12(Protocol #Orchid Virus Testing) $24 81 Cymbidium mosaic virus Potato virus Y Maize dwarf mosaic virus Tomato spotted wilt virus Melon necrotic spot virus Zucchini yellow mosaic virus * Import delays of around 7 days apply for most tests Plant Diagnostic Services -

2016 Plant Virus Diagnostics Catalogue

2015-12-7for the detection of the first 10 virus species highlighted below It is our pleasure to present the new Plant Virus Diagnostics Catalogue 201 6 Please contact us directly (plantvirusdsmz de) for diagnostic and virological services that you do not find in our current catalogue For the DSMZ Plant Virus Team Stephan Winter Wulf Menzel

2017-2-28Tomato in Thailand Survey of tospovirues infecting tomato pepper and cucurbits in Thailand Production of monoclonal antibodies and development of immunoassays for detection of tospoviruses 2011 2011-2013 2013-2016 2015-2017 Development of efficient inoculation protocol for TNRV and CaCV resistance screening in tomato

Potato is widely grown in the EU as reported in the pest categorisation of non‐EU viruses and viroids of potato (EFSA PLH Panel 2020) 3 4 3 2 Climatic conditions affecting establishment Except for those conditions affecting survival of the host plants no eco‐climatic constrains exist for the PVM isolates categorised here

Pathoscreen Pathoscreen kits for the diagnosis of plant pathogens are ELISA test kits that contain all the materials necessary to perform an ELISA protocol Most importantly our kits include precoated plates which save time and offer extraordinary stability Kits are available in

Detection and epidemiology of pospiviroids (DEP) Project slide Final report Strategies for Ambrosia artemisiifolia control (AMBROSIA) Project slide Final report Development and validation of innovative diagnostic tools for the detection of fire blight (Erwinia amylovora) (ERWINDECT)Project slide

(3) Diagnostic methods for Potato wart disease a Contribute to validation of Dutch Taqman PCR method for pathotype-1 b Investigate the use of pyrosequencing for discriminating pathotypes and use to develop real-time PCR assays c Take part in harmonization of sampling detection and identification protocols

Diagnostic protocol for the detection and identification of 'Candidatus Liberibacter solanacearum' in carrot seeds (DNA extraction method real-time PCR and conventional PCR) Detection of PepMV by RT-Q PCR in tomato seed and leaves: no: extraction molecular: Detection of Potato spindle tuber viroid (PSTVd) and/or Tomato chlorotic dwarf

2020-8-1Pospiviroid belongs to the Pospiviroidae family on the basis of biochemical biological and structural properties which have been classified The genus Pospiviroid contains nine viroid species: Potato spindle tuber viroid (PSTVd) Tomato chlorotic dwarf viroid (TCDVd) Mexican papito viroid (MPVd) Tomato planta macho viroid (TPMVd) Chrysanthemum stunt viroid (CSVd) Citrus exocortis

Grape

2020-7-17packed in a manner that prevents infestation by tomato potato psyllid and undergo a 2% or 600 unit inspection and be found free of tomato potato psyllid or fumigated with 1000 g/kg active constituent methyl bromide at: 10C ‐ 10 9C 56g/m 3 for 2 hours or 11C ‐ 15 9C 48g/m 3 for 2 hours or 16C ‐ 20 9C 40g/m 3 for 2

2015-3-22TRV causes disease in various plants (e g potato tomato and tobacco) for which it was classified as a controlled quarantine virus in Korea This study aimed to develop specific primer sets for the rapid detection of TRV Two RT-PCR primer sets were

ORIGINAL ARTICLE Detection of Tobacco mosaic virus and Tomato mosaic virus in pepper and tomato by multiplex RT–PCR S Kumar1 A C Udaya Shankar1 S C Nayaka1 O S Lund2 and H S Prakash1 1 Department of Studies in Biotechnology University of Mysore

Rosemarie W Hammond Shulu Zhang Development of a rapid diagnostic assay for the detection of tomato chlorotic dwarf viroid based on isothermal reverse-transcription-recombinase polymerase amplification Journal of Virological Methods 10 1016/j jviromet 2016 06 013 236 (62-67) (2016)

and are therefore not suitable for routine diagnostic work As an alternative Elphinstone et al (1996) found that sample dilution and enrichment in SMSA broth followed by the use of a two-stage nested PCR protocol was effective during pathogen detection in potato tuber homogenate The sensitivity of methods based on tomato bioassay

Potato cyst nematodes have a narrow host range The plants on which they are found (potato tomato and eggplant) are a good indicator for species identification in the case of PCN If round yellow females are found on the roots of potato plants during the growing season the species is

2017-1-26Detection and Epidemiology of Pospiviroids Pospiviroids are plant pathogens composed only of infectious RNA molecules which are both latent on many ornamentals and harmful for cultivated plant species like tomato potato sweet pepper citrus or chrysanthemum Throughout the world these pathogens cause sporadic outbreaks on susceptible annual